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Time-lapse imaging was carried out at 50 Hz and 20 ms exposure at 37 C on Roper microscope (Roper Scientific) equipped with an iLas2 double-laser illuminator, a CFI Apo TIRF 100 1.49 NA objective (Nikon) and an Evolve512 delta EMCCD camera (Photometrics, model no

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Saliba, A

These improvements in senescence markers and the inflammatory microenvironment underscore FOXO4-DRIs potential to rejuvenate testicular function by clearing senescent cells and mitigating their detrimental effects